Inhibition of xanthine oxidase by the aldehyde oxidase inhibitor raloxifene: Implications for identifying molybdopterin nitrite reductases
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چکیده
منابع مشابه
Potent inhibition of human liver aldehyde oxidase by raloxifene.
The selective estrogen receptor modulator, raloxifene, has been demonstrated as a potent uncompetitive inhibitor of human liver aldehyde oxidase-catalyzed oxidation of phthalazine, vanillin, and nicotine-Delta1'(5')-iminium ion, with K(i) values of 0.87 to 1.4 nM. Inhibition was not time-dependent. Raloxifene has also been shown to be a noncompetitive inhibitor of an aldehyde oxidase-catalyzed ...
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Background Any factor like diabetes that changes the oxidant/antioxidant balance in favor of oxidants could possibly disrupt the physiologic function of ovaries. So, we have investigated the effect of vitamin E and Se supplements on antioxidant defense in ovaries of diabetic rats with focus on Xanthine dehydrogenase/oxidase (XDH/XO) and Aldehyde oxidase (AO) activities. MaterialsAndMethods This...
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Various dietary flavonoids were evaluated in vitro for their inhibitory effect on xanthine oxidase, which has been implicated in oxidative injury to tissue by ischemia-reperfusion. Xanthine oxidase activity was determined by directly measuring uric acid formation by HPLC. The structure-activity relationship revealed that the planar flavones and flavonols with a 7-hydroxyl group such as chrysin,...
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Methods have been devised to examine the spectral properties and state of reduction of the pterin ring of molybdopterin (MPT) in milk xanthine oxidase and the Mo-containing domain of rat liver sulfite oxidase. The absorption spectrum of the native pterin was visualized by difference spectroscopy of each protein, denatured anaerobically in 6 M guanidine hydrochloride (GdnHCl), versus a sample co...
متن کاملHydroxylamine Inhibition of Xanthine Oxidase
In the course of investigations regarding the effects of various chemical agents on neoplast,ic purine metabolism, it was observed that hydroxylamine prevents the accumulation of uric acid or allantoin that normally occurs upon incubation of crude tissue homogenates. Subsequent studies, reported in this paper, demonstrate that hydroxylamine is a competitive inhibitor of xanthine oxidase activit...
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ژورنال
عنوان ژورنال: Nitric Oxide
سال: 2014
ISSN: 1089-8603
DOI: 10.1016/j.niox.2013.12.010